Introduction to Cryo-electron Microscopy Cryo-electron microscopy (Cryo-EM) is an imaging technique used to visualize the structure of macromolecules, such as proteins and viruses, at near-atomic resolution. Cryo-EM uses a method called electron cryomicroscopy, which involves rapidly freezing a sample at very low temperatures to minimize structural damage from the electron beam. The sample is then imaged using an electron microscope, and the resulting images are processed using specialized so...